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Radiation Protection Dosimetry Advance Access published online on January 17, 2007
Radiation Protection Dosimetry, doi:10.1093/rpd/ncl443
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Micros 2005 Special Issue

RADIATION DAMAGE TO DNA-PROTEIN SPECIFIC COMPLEXES: ESTROGEN RESPONSE ELEMENT-ESTROGEN RECEPTOR COMPLEX

Viktorie Stísová 1, Stephane Goffinont 2, Melanie Spotheim-Maurizot 2, and Marie Davídková 3 *

1 Department of Radiation Dosimetry, Nuclear Physics Institute AS CR, Na Truhlárce 39/64, 180 86, Praha 8, Czech Republic; Department of Dosimetry and Application of Ionizing Radiation, Faculty of Nuclear Sciences and Physical Engineering, CTU Prague, Brehová 7, 115 19, Praha 1, Czech Republic
2 Centre de Biophysique Moléculaire CNRS, rue Charles Sadron, 45100 Orléans Cedex 2, France
3 Department of Radiation Dosimetry, Nuclear Physics Institute AS CR, Na Truhlárce 39/64, 180 86, Praha 8, Czech Republic

* To whom correspondence should be addressed.
Marie Davídková, E-mail: davidkova{at}ujf.cas.cz


  Abstract

The exposure of a DNA-protein regulatory complex to ionising radiation induces damage to both partner biomolecules and thus can affect its functioning. Our study focuses on a complex formed by the estrogen response element (ERE) DNA and the recombinant human estrogen receptor alpha (ER), which mediates the signalling of female sex hormones, estrogens. The method of native polyacrylamide retardation gel electrophoresis is used to study the stability of the complex under irradiation by low LET radiation (60Co gamma rays) and the ability of the separately irradiated partners to form complexes. The relative probabilities of ERE DNA strand breakage and base damages as well as the probabilities of damages to the ER binding domain are calculated using the Monte Carlo method-based model RADACK.


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