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Radiation Protection Dosimetry Advance Access published online on December 19, 2006
Radiation Protection Dosimetry, doi:10.1093/rpd/ncl434
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

SCIENTIFIC AND TECHNICAL PAPER

RADIATION-INDUCED {gamma}-H2AX IN MAMMALIAN CELLS IRRADIATED WITH A SYNCHROTRON X-RAY MICROBEAM

N. Usami 1 *, M. Maeda 2, K. Eguchi-Kasai 3, H. Maezawa 4, and K. Kobayashi 1

1 Photon Factory, High Energy Accelerator Research Organization (KEK), Tsukuba 305-0801, Japan
2 School of High Energy Accelerator Science, Graduate University of Advanced Studies, Tsukuba 305-0801, Japan
3 National Institute of Radiological Sciences, Chiba 263-8555, Japan
4 Faculty of Medicine, University of Tokushima, Tokushima 770-8509, Japan

* To whom correspondence should be addressed.
N. Usami, E-mail: noriko.usami{at}kek.jp


  Abstract

In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 µm with the focusing optics and 5 µm square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by {gamma}-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size.


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