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Radiation Protection Dosimetry Advance Access published online on November 28, 2006
Radiation Protection Dosimetry, doi:10.1093/rpd/ncl420
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Micros 2005 Special Issue

DNA-REPAIR PROTEIN DISTRIBUTION ALONG THE TRACKS OF ENERGETIC IONS

A. Hauptner 1 *, R. Krücken 1, C. Greubel 2, V. Hable 2, G. Dollinger 2, G. A. Drexler 3, M. Deutsch 3, R. Löwe 3, A. A. Friedl 3, S. Dietzel 4, H. Strickfaden 4, and T. Cremer 4

1 Physik Department E12, TU-München, James-Franck-Strasse, 85748 Garching, Germany
2 Institut für angewandte Physik und Messtechnik (LRT2), UniBW-München, Werner-Heisenberg-Weg 39, 85577 Neubiberg, Germany
3 Strahlenbiologisches Institut, LMU-München, Schillerstrasse 42, 80336 München, Germany
4 Department Biologie II, LMU-München, Grosshadernerstrasse 2, 82152 Planegg-Martinsried, Germany

* To whom correspondence should be addressed.
A. Hauptner, E-mail: andreas.hauptner{at}ph.tum.de


  Abstract

A simple model of homogenous chromatin distribution in HeLa-cell nuclei suggests that the track of an energetic ion hits 30 nm chromatin fibers with a mean distance of 0.55 µm. To test this assumption, living HeLa-cells were irradiated at the irradiation setup of the ion microprobe SNAKE using the ion beams provided by the Munich 14 MV tandem accelerator. After irradiation, the distribution of 53BP1 protein foci was studied by immunofluorescence. The observed 53BP1 distribution along the tracks of 29 MeV 7Li ions and 24 MeV 12C ions differed significantly from the expectations resulting from the simple chromatin model, suggesting that the biological track structure is determined by cell nuclear architecture with higher order organisation of chromatin.


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