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Radiation Protection Dosimetry Advance Access originally published online on January 24, 2007
Radiation Protection Dosimetry 2006 122(1-4):205-206; doi:10.1093/rpd/ncl391
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

1H MRS signals from glutathione may act as predictive markers of apoptosis in irradiated tumour cells

S. Grande1, C. Giovannini2, L. Guidoni1, A. M. Luciani1, A. Palma1, A. Rosi1, O. Sapora3 and V. Viti1,*

1 Department of Technology and Health and INFN Sanità Group, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy
2 National Centre for Food Quality and Risk Assessment, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy
3 Department of Environment and Primary Prevention, Istituto Superiore di Sanità, Viale Regina Elena 299, Roma, Italy

* Corresponding author: viti{at}iss.it


  Abstract

Inhibition of apoptosis in tumour cells may depend on intracellular reduced glutathione (GSH) level. In this work, GSH levels were studied by 1H MRS in MCF-7 and HeLa cells, characterised by a different radiosensitivity. Annexin-V test showed that the fraction of apoptotic HeLa cells after irradiation is much higher than in control, although MCF-7 cells did not show a significant apoptosis. MRS signals from GSH (G) show lower intensity in HeLa with respect to MCF-7 cells; the opposite is true for free glutamic acid [glu (g)]. After irradiation, the G/g ratio decreases in MCF-7, although remaining approximately constant in HeLa cells. Buthionine sulfoximine (BSO) treated MCF-7 cells show an increase in the percentage of apoptotic cells; in parallel, G/g ratio behaves as in HeLa. This study indicates that GSH level may act as predictive marker of apoptosis by irradiation.


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