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Radiation Protection Dosimetry Advance Access originally published online on November 28, 2006
Radiation Protection Dosimetry 2006 122(1-4):160-162; doi:10.1093/rpd/ncl393
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Alpha-particle radiobiological experiments using thin CR-39 detectors

K. F. Chan1, S. Y. M. Siu2, K. E. McClella2, A. K. W. Tse2, B. M. F. Lau1, D. Nikezic3, B. J. Richardson2, P. K. S. Lam2, W. F. Fong2 and K. N. Yu1,*

1 Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong
2 Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong
3 Faculty of Sciences, University of Kragujevac, Serbia and Monte Negro

* Corresponding author: peter.yu{at}cityu.edu.hk


  Abstract

The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 µm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37°C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.


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