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Radiation Protection Dosimetry Advance Access originally published online on January 17, 2007
Radiation Protection Dosimetry 2006 122(1-4):100-105; doi:10.1093/rpd/ncl442
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

INVITED PAPER

Modification of DNA radiolysis by DNA-binding proteins: structural aspects

Marie Davídková1,*, Viktorie Stísová1,3, Stephane Goffinont2, Nathalie Gillard2, Bertrand Castaing2 and Melanie Spotheim-Maurizot2

1 Department of Radiation Dosimetry, Nuclear Physics Institute AS CR, Na Truhlárce 39/64, 180 86, Praha 8, Czech Republic
2 Centre de Biophysique Moléculaire CNRS, rue Charles Sadron, 45100 Orléans Cedex 2, France
3 Department of Dosimetry and Application of Ionizing Radiation, Faculty of Nuclear Sciences and Physical Engineering, CTU Prague, Brehová 7, 115 19, Praha 1, Czech Republic

* Corresponding author: davidkova{at}ujf.cas.cz


  Abstract

Formation of specific complexes between proteins and their cognate DNA modulates the yields and the location of radiation damage on both partners of the complex. The radiolysis of DNA–protein complexes is studied for: (1) the Escherichia coli lactose operator–repressor complex, (2) the complex between DNA bearing an analogue of an abasic site and the repair protein Fpg of Lactococcus lactis. Experimental patterns of DNA damages are presented and compared to predicted damage distribution obtained using an improved version of the stochastic model RADACK. The same method is used for predicting the location of damages on the proteins. At doses lower than a threshold that depends on the system, proteins protect their specific binding site on DNA while at high doses, the studied complexes are disrupted mainly through protein damage. The loss of binding ability is the functional consequence of the amino-acids modification by OH· radicals. Many of the most probably damaged amino acids are essential for the DNA–protein interaction and within a complex are protected by DNA.


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