Review of translocations detected by FISH for retrospective biological dosimetry applications
1 Health Protection Agency, Radiation Protection Division (HPA), Chilton, Didcot, Oxon OX11 0RQ, UK
2 Radiation and Nuclear Safety Authority (STUK), Laippatie 4, 00881 Helsinki, Finland
3 Department of Toxicogenetics, Leiden University Medical Centre (LUMC), Wassenaarseweg 72, 2333AL Leiden, The Netherlands
4 Bundesamt für Strahlenschutz (BfS), Ingoldstaedter Landstrasse 1, 85764 Oberschleissheim, Germany
5 Universitat Autonoma de Barcelona (UAB), Campus Universitari, 08193 Bellaterra, Spain
6 Institut de Radioprotection et Sûreté Nucléaire (IRSN), BP17, 92262 Fontenay-aux-Roses Cedex, France
7 Westlakes Research Institute (WRI), Westlakes Science Park, Moor Row, Cumbria CA24 3JY, UK
* Corresponding author: alan.edwards{at}hpa-rp.org.uk
Received October 26, 2004, amended December 13, 2004, accepted December 18, 2004
Several European laboratories have combined their research efforts to arrive at a consensus view on using fluorescence in situ hybridisation (FISH) for retrospective dosimetry. The aim of this review is to report these views and to highlight some areas where further work is needed. Translocations in the stable cells should be measured only in the cells that contain the full complement of the painted material. Two-way and one-way translocations should be combined with equal weight. The control level of translocations has a strong dependence on age, which has now been measured and the system has been calibrated. In conclusion, the technique works and a lifetime dose to the bone marrow from low–linear energy transfer radiation of 0.5 Gy above normal background levels can be measured for any individual. The main application is considered to provide an independent verification of lifetime doses to individuals who might form a part of an epidemiological study.
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